This study constructed a chromosome-level genome assembly of Eucalyptus urophylla × Eucalyptus grandis (E. urograndis) clone DH32-29 using BGI-SEQ500, PacBio-SMRT, and Hi-C technologies, with a genome size of 592.09 Mb and a scaffold N50 of 58.62 Mb.

Using sequencing technologies such as PacBio HiFi long reads and Omni-C, the researchers successfully constructed a haplotype-resolved reference genome (v4.0) for Eucalyptus grandis TAG0014, showing significant improvements in contiguity and completeness.

Researchers generated a high-quality chromosome-level genome assembly of Eucalyptus globulus Labill. with a genome size of 556.98 Mb, a contig N50 of 37.93 Mb, and a scaffold N50 of 54.03 Mb, providing a valuable resource for elucidating molecular mechanisms.